Protein A/G/L, Recombinant
439.92 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-P9102-17D
Supplier
US Biologicals
Size
1 mg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Protein A/G/L is a genetically engineered protein that combines the IgG binding profiles of all Protein A, Protein G and Protein L. It is a gene fusion product. Recombinant fusion protein A/G/L contains 6x His-tag on the N-terminus with five Ig-binding regions of protein L, five IgG binding domains from Protein A , and three Ig-binding region of protein G. Cell wall binding regions, albumin binding regions and other non-specific binding regions have all been eliminated from the fusion protein to ensure the maximum specific IgG binding. 6x His-tag on N-terminus can be used for affinity purification or for protein A/G/L detection using anti-His-tag antibody. Protein A/G/L binds to all IgG subclasses from various mammalian species, including all IgGs that bind to Protein A, Protein G, and Protein L, individually, making it the ideal choice for purification of all kinds of polyclonal or monoclonal IgG antibodies. Source: E. coli THEORETICAL PI: 4.76 SPECIFICITY: Under optimal conditions, 1mg protein A/G/L will bind approximately 5-15mg human IgG. Optimal binding of Protein A/G/L to antibodies occurs at pH 5.0 to 8.0 and can be eluted over a pH range of 2.5 to 3.0. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Recombinant, E. coli |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 101 |
Product Properties
| Storage | -20°C |
| Grade | Highly Purified |
| Purity | ≥95% (SDS-PAGE and HPLC). Endotoxin: ≤0.1EU/mg |
| Form | Supplied as a lyophilized powder. Reconstitute with sterile ddH2O to 10mg/ml. |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
