Protein A/G, Recombinant (Agarose)
511.67 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-P9102-45C
Supplier
US Biologicals
Size
1ml
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Protein A/G Sepharose is prepared by covalently coupling recombinant Protein A/G (contains five Ig-binding regions of protein A and three Ig-binding regions of protein G, to 6% cross-linked sepharose beads. The coupling was optimized to give a high binding capacity for IgG. The capacity of IgG binding could be greater than 20mg of rabbit IgG per ml of wet gel. Protein G is a small globular cell surface protein produced by Streptococcus sp. It is composed of two or three nearly identical domains of 55 amino acids each. It binds to Fc fragment of IgG with high affinity. Protein G will bind IgGs from many species including all human and mouse subclasses. It does not recognize IgM, IgA, IgE or serum albumin. Protein G binds IgG from rat,, but substantially more weakly than IgG from other species. Recombinant Protein G is a highly stable surface receptor from Streptococcus sp. Lancefield Group G, produced in Escherichia coli, which is capable of binding the Fc portion of immunoglobulins, especially IgGs, from a large number of species (Boyle and Reis, 1987). Each protein G molecule can bind 2 molecules of IgG, allowing the formation of a precipitate. Protein G may be coupled to a wide variety of reporter molecules including fluorescent dyes, enzyme markers, biotin, colloidal gold and radioactive iodine without affecting the antibody binding site. Applications: Purification of monoclonal and polyclonal antibodies. Other applications not tested. Binding Capacity: Binding capacity greater than 20mg/ml of wet gel. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Recombinant, E. coli |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | 4°C Do Not Freeze |
| Grade | Molecular Biology Grade |
| Purity | Purified |
| Form | Supplied as a 50% slurry in 20% Ethanol/H2O. >1.5mg Protein A/G/ml Sepharose beads. |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
