Protein A, Recombinant, S. aureus, 50mg/ml
232.69 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-P9102-11
Supplier
US Biologicals
Size
5 mg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Protein A is a highly stable surface receptor produced by Staphylococcus aureus, which is capable of binding the Fc portion of immunoglobulins, especially IgGs, from a large number of species (Boyle and Reis, 1987). Each protein A molecule can bind 2 molecules of IgG, allowing the formation of a precipitate (Sjöholm, 1975). Protein A may be coupled to a wide variety of reporter molecules including fluorescent dyes, enzyme markers, biotin, colloidal gold and radioactive iodine without affecting the antibody binding site. These conjugates may be used to track immunoglobulins in histochemical, Western Blot and ELISA. Alternatively, Protein A may be immobilized onto a solid support to facilitate the purification and recovery of either polyclonal or monoclonal immunoglobulins. Immobilized protein A has recently been applied therapeutically to treat a variety of humoral diseases (Watson et al, 1989). Applications: Immunologically active enzyme conjugates, ELISA’s, dot blot and western immunoblotting techniques, affinity purification of polyclonal and monoclonal antibodies and immunoprecipitation. Specificity: Protein A will bind the Fc portion of human IgG subclasses, IgM, IgA and IgE; and mouse IgG1 (weakly), IgG2a and IgG2b. Protein A also binds IgGs from other laboratory and domestic animals, including monkey, rabbit, pig, guinea pig, dog and cat. Purity (HPLC): ≥95% Activity (binding to human IgG): ≥95% Form: Supplied as a sterile-filtered solution 50mg/ml solution in Molecular Biology grade water. Molecular Weight: 42kD Composition: Protein A is a monomeric protein lacking cysteine residues. Extinction Coefficient: E280(1%): 1.4 Stability Range: pH 1.0-12.0 Isoelectric Point: 4.85-5.10 Storage & Stability: Protein A may be stored at -20°C in working aliquots; no apprecialbe decay in Fc-binding activity has been detected after six months of storage. |
| Source Antigen | Recombinant, S. aureus |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 42 |
Product Properties
| Storage | -20°C |
| Grade | Molecular Biology Grade |
| Purity | Activity (binding to human IgG): ≥95% |
| Form | Supplied as a sterile-filtered solution 50mg/ml solution in Molecular Biology grade water. |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
