Protein A (Sepharose)
634.23 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-P9102-04A
Supplier
US Biologicals
Size
5ml
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Protein A and Protein G bind specifically to Fc regions of many mammalian immunoglobulins. Protein A and Protein G conjugates are commonly used as affinity adsorbents to purify immunoglobulins (antibodies) and immunoglobulin subtypes from serum, hybridoma ascites fluids, tissue culture supernatants, and other biological fluids. These reagents are also commonly used to capture immune complexes generated in immunoprecipitation experiments. Because Protein A and Protein G vary widely in their abilities to bind individual immunoglobulins, consult the table below before proceeding with your application. It should be noted that the Fc binding characteristics of naturally produced Protein A and Protein G are identical to their recombinant counterparts. Protein-A/Sepharose® ratio: ~2.7mg Protein A per ml beads Binding capacity: ~22mg Rabbit IgG per ml beads Protein A Molecular Weight: 42kD (native) Protein A Ig Binding Sites: 4 per molecule Albumin Binding Sites/molecule: none Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C. Freezing will damage or destroy its performance. |
| Source Antigen | Recombinant |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | 4°C Do Not Freeze |
| Grade | Highly Purified |
| Purity | ~98% (SDS-PAGE) |
| Form | Supplied as a 50% suspension (5 ml Protein A-Sepharose® 4B + 5 ml PBS) in 10 mM phosphate-buffered saline, pH 7.4, containing 0.1% Proclin |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
