Protein G, Recombinant
502.78 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-209158
Supplier
US Biologicals
Size
1 mg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Protein G is a bacterial cell wall protein isolated from group G Streptococci. Native Protein G contains two IgG-binding domains and sites for albumin and cell surface binding. The albumin and cell surface binding domains have been eliminated from purified Recombinant Protein G to reduce nonspecific binding and, therefore, can be used to separate IgG from crude samples. Optimal binding occurs at pH 5, although binding is also good at pH 7.0-7.2. Protein G has greater affinity than Protein A for most mammalian IgGs, it may be used for the purification of mammalian IgGs that do not bind well to Protein A. Protein G does not bind to human IgM, IgD and IgA. Native protein is ~65kD. It contains two albumin binding domains at the N-terminus and 3 IgG binding domains (~55aa each) at the C-terminus. Recombinant protein G does not contain albumin binding domains. Differences in binding characteristics between Protein A and Protein G may be explained by the differing compositions in the IgG-binding sites of each protein, and sequence differences between Protein A and Protein G. Variations in isolation and purifications methods for Protein G may affect IgG binding, partially because there are differing numbers of IgG-binding sites on various sources of Protein G. Source: Recombinant protein corresponding to Protein G from Staphylococcus Group G, expressed in E. coli. Molecular Weight: ~23kD, migrates to ~32kD protein Binding Capacity: 1mg binds 10-15mg of human IgG. Binding of other species may be different. Endotoxin: <0.5EU/mg protein Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Recombinant |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 2332 |
Product Properties
| Storage | -20°C |
| Grade | Highly Purified |
| Purity | Highly Purified (~95%) |
| Form | Supplied as a lyophilized powder in salt free form. Reconstitute with sterile ddH2O or PBS. |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
