Protein G, Recombinant, Streptococcus sp. (Biotin)
433.79 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-P9102-45F
Supplier
US Biologicals
Size
100 µg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Protein G binds to the constant region of many species of immunoglobulin G. Protein G can be used to detect, quantify and purify IgG antibodies and antibody/antigen complexes. Recombinant Protein G contains only IgG binding domains. The albumin-binding domain as well as cell wall and cell membrane binding domains have been removed to ensure the maximum specific IgG binding capacity. The 6-His-tag on N-terminus can be used for affinity purification or protein G detection using anti-His-tag antibodies. Protein G binds to all IgG subclasses from human, mouse and rat species. It also binds to total IgG from guinea pig, rabbit, goat, cow, sheep, and horse. The Protein G-Biotin is using purified recombinant protein G biotinylated with ~2 biotins per protein G molecule. Source: E.coli Endotoxin level: ≤0.1EU/mg Specificity: Under optimal conditions, 1mg protein G will bind approximately 5mg human IgG. Optimal binding of Protein G to antibodies occurs at pH 5.0 to 6.0 and can be eluted over a pH range of 2.5 to 3.0. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Stable for at least 6 months at -20°C. Reconstitute with sterile ddH2O, aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Recombinant |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 26.1 |
Product Properties
| Storage | -20°C |
| Grade | Purified |
| Purity | ≥98% (SDS-PAGE and HPLC) |
| Form | Supplied as a lyophilized powder. Reconstitute in ddH2O to 5 mg/ml. |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
