PsuI (XhoII)
410.43 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-P9181
Supplier
US Biologicals
Size
500U
Product Overview
Product Properties
Target Gene
Product Overview
| Description | 5′-Pu↓G A T C Py-3′ 3′-Py C T A G↑Pu-5′ Source: Pseudomonas stutzeri Mck28-pH52 Concentration: 10u/ul Unit Definition: One unit is defined as the amount of PsuI required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of recommended reaction buffer. Form: Supplied as a liquid in 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA, 50% glycerol. Supplied with: R1625: Restriction Enzyme Buffer A, 10X: Dilute to 1X for use. 1X buffer composition is 33mM Tris-acetate pH 7.9 at 37°C, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. Supplied with R1625-01 Restriction Enzyme Buffer B, 10X: Dilute to 1X for use. 1X buffer composition is 10mM Tris-HCl pH 7.5, 10mM MgCl2, 0.1mg/ml BSA. Thermal Inactivation: PsuI is inactivated by incubation at 80°C for 20 minutes. Diluent Buffer: 10mM Tris-HCl, pH 7.4, 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after a 80-fold overdigestion with PsuI (5u/μg lambda DNA x 16 hours). Ligation and Recleavage (L/R) Assay: The ligation and recleavage assay was replaced with LO test after validating experiments showed LO test ability to trace nuclease and phosphatase activities with sensitivity that is higher than L/R by a factor of 100. Labeled Oligonucleotide (LO) Assay: No detectable degradation of single-stranded or double- stranded labeled oligonucleotides occurred during incubation with 10 units of PsuI for 4 hours. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at -20°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | |
| Reactivity | |
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| Molecular Weight |
Product Properties
| Storage | -20°C |
| Grade | Molecular Biology Grade |
| Purity | |
| Form | |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
