Purification Media, BioSeparation™ Affinity, Protein G (Agarose)
535.79 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-P9100-85
Supplier
US Biologicals
Size
1ml
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Protein G originated from Streptococci is a monomeric protein with a molecular weight of about 50,000kD. Protein G binds with high affinity to the Fc region of most classes and subclasses of immunoglobulins from mouse, rabbit, goat and human. Due to this binding ability, Protein G is commonly used in the purification and recovery of either polyclonal or monoclonal immunoglobulins. Recombinant Protein G used in Protein G beads is a genetically engineered form of Protein G. Non-essential regions have been removed while leaving the IgG binding sites intact. The recombinant Protein G has been immobilized on Sepharose beads in order to create a versatile product for both the purification of various mammalian immunoglobulins and for isolating immune complexes by immunoprecipitation (IP). Matrix: SepharoseTM CL-4B Coupling Method: TargetLock Protein G Density: 3-4mg/ml of gel Binding Capacity: ~4-8mg rabbit IgG per ml of beads Mean Bead Size: 40-165um Bead Structure: Highly cross-linked spherical agarose, 4% Max Back Pressure: 0.3MPa, 3 bar Max. Flow Rates: 4ml/min/cm2 Recommended Flow Rate: 1-3 ml/min/cm2 Stability of the Matrix: pH 2-11. Storage: Store gel refrigerated at 4°C in PBS pH 7.4 containing 0.1% sodium azide as a preservative, or PBS pH 7.4 containing 20% ethanol. |
| Source Antigen | Recombinant |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | 4°C |
| Grade | Molecular Biology Grade |
| Purity | |
| Form | |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
