Reverse Transcriptase, M-MLV
629.59 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-R1991-02
Supplier
US Biologicals
Size
100KU
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Source: E.coli Unit Definition: One unit of the enzyme incorporates I nmole dTTP into acid-precipitable material in 10 minutes at 37°C, using poly (A): oligo dT as a template:primer. Quality Assurance: Free of endonucleases and exonucleases Components: 1. M-MLV Reverse Transcriptase (200units/ul) 2. 5X reaction buffer Quality Control: Nonspecific Endonuclease/Nickase Activity: None detected after 14-16 hour incubation of 100units of enzyme with 300ng of supercoiled plasmid DNA, analyzed on agarose gel. Exonuclease Activity: None detected after 14-16 hour incubation of 100units of enzyme with 40ng of 32P-labeled Sau3A fragments of pUC19, analyzed by PAGE. Protease Activity: None detected after 14-16 hour incubation of 1200units of enzyme with 1ug of protease substrate, analyzed by fluorescence. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge` the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | E.coli |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | -20°C |
| Grade | |
| Purity | |
| Form | Supplied as a liquid in (5X) 250mM Tris HCl, pH 8.3, 375mM KCl, 15mM MgCl2, 50mM DTT. |
| Reactivity Life | 6 months |
| Note | For reserch purpose only |
Target Gene
NA
