Reverse Transcriptase, M-MLV (M-MLV-RT) Recombinant
419.70 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-R1991-01
Supplier
US Biologicals
Size
2.5KU
Product Overview
Product Properties
Target Gene
Product Overview
| Description | MMLV (Molony Murine Leukemia Virus) Reverse Transcriptase is a DNA polymerase that synthesizes a complementary DNA strands from single-stranded RNA, DNA, or an RNA-DNA hybrid as a template. This recombinant enzyme was purified from E.coli. which carried a modified MMLV-RT gene. Compared to AMV Reverse Transcriptase, this enzyme has a much weaker 5′-3′ ribonuclease H activity, which allows the syntesis of longer cDNAs (>7kb). Unit Definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 1nmol of deoxyribonucleotide into acid-insoluble forms in 10 minutes at 37oC, using poly(A)-oligo(dT)12-18 as the template-primer. Standard cDNA Synthesis Conditions: 50mM Tris-HCl (pH8.3), 75mM KCl, 3mM MgCl2, 10mM DTT, 1.0mM each dATP, dGTP, dCTP, and dTTP, 0.2ug radom hexamer,1-5ug RNA, 200units M-MLV RT. The reaction volume was 20µl and the incubation was 45 min at 42oC. 5X Reaaction Buffer: 250mM Tris-HCl (pH8.3), 375mM KCl, 15mM MgCl2, and 50mM DTT. Applications: 1. 1st strand cDNA synthesis. 2. Primer extension. 3. RT-PCR. Stability: Stable for 5 days at 10oC, for longer period of time store at -20oC. Please avoid freeze-thaw cycles. |
| Source Antigen | Recombinant, E. coli |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | -20°C |
| Grade | |
| Purity | |
| Form | Supplied as a liquid in 50mM Tris-HCl, 0.1M sodium chloride, 0.1% Triton X-100, 2mM DTT, 0.1mM EDTA and 50% glycerol. |
| Reactivity Life | 6 months |
| Note | For reserch purpose only |
Target Gene
NA
