RNA Polymerase, T7
469.24 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-R2049-05
Supplier
US Biologicals
Size
5000U
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Storage Buffer: 20mM Potassium Phosphate (pH 7.9), 1mM DTT, 0.1mM EDTA, 100mM NaCl and 50% glycerol. Reaction Buffer (5X): 200mM Tris-HCl (pH 7.9 at 25°C), 30mM MgCl, 50mM NaCl, 10mM spermidine. Unit Definition: One unit is the amount of enzyme that incorporates I nmole of [3H] GMP into acid-insoluble fraction in 1 hour at 37°C and pH 8. Purity: Nuclease activity was not detected in any of the following cases, as judged from the intact gel electrophoresis: 1. After incubation of 1ug of λ DNA-Hind III fragments with 500 units of enzyme for 24 hours at 37°C 2. After incubation of 1ug of pBR322 DNA with 500 units of enzyme for 24 hours at 37°C 3. After incubation of 1ug of 16S and 23S rRNA with 500 units of enzyme for 24 hours at 37°C This enzyme was more than 95% homogeneous as judged from SDS-polyacrylamide gel electrophoresis. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | E. coli |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | -20°C |
| Grade | Highly Purified |
| Purity | |
| Form | |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
