sCD27 Ligand, Recombinant, Human (TNFSF7, CD70)
396.23 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-143490
Supplier
US Biologicals
Size
10 µg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | CD27 Ligand, a type II transmembrane protein, is a member of the TNF superfamily. It is expressed on activated T and B lymphocytes as well as NK cells. CD27L and its receptor (CD27) regulate the immune response by promoting T-cell expansion and differentiation, as well as NK enhancement. CD27 signaling can act as a co-stimulatory effector to sustain the survival of CD8+ T cells, primarily by inducing increased expression of the IL-2 gene. Full length human CD27L is a 193 amino acid protein, consisting of a 17 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 155 amino acid extracellular domain. Human soluble CD27L corresponds to the 155 amino acid extracellular domain of the full length CD27L protein. Recombinant human sCD27L contains the extracellular domain plus an N-terminal His-Tag. Biological Activity: Determined by its ability to stimulate human IL-8 production by human PBMC using a concentration range of 10-25ng/ml. Note: Results may vary with PBMC donors. Amino Acid Sequence: HHHHHHHHPS PGGSGGQRFA QAQQQLPLES LGWDVAELQL NHTGPQQDPR LYWQGGPALG RSFLHGPELD KGQLRIHRDG IYMVHIQVTL AICSSTTASR HHPTTLAVGI CSPASRSISL LRLSFHQGCT IASQRLTPLA RGDTLCTNLT GTLLPSRNTD ETFFGVQWVR P Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 6 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | CHO cells |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | -20°C |
| Grade | Highly Purified |
| Purity | ≥95% by SDS-PAGE gel and HPLC analyses. Endotoxin: ≤0.1ng/ug of protein (<1EU/ug). |
| Form | Supplied as a lyophilized powder from 20mM sodium citrate, pH 3.0. Reconstitute with sterile ddH2O to a concentration of 0.1-1mg/ml.. DO NOT VORTEX. Further dilutions can be made in a buffer with 0.1% HSA or BSA. |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
