T4 DNA Ligase, 5u/ul, E. coli
434.80 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-301289
Supplier
US Biologicals
Size
250U
Product Overview
Product Properties
Target Gene
Product Overview
| Description | T4 DNA ligase catalyzes the formation of phosphodiester bonds between 5’-phosphate and 3’-hydroxy ends of double-stranded DNA. Single stranded nicks in double-stranded DNA are also closed by T4 DNA ligase. T4 DNA ligase needs ATP as co-factor. Source: T4 DNA Ligase, from E. coli. Applications: Suitable for use in Ligate double-stranded nucleic acid fragment. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Source Antigen | E. coli |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight |
Product Properties
| Storage | -70°C |
| Grade | |
| Purity | Tested for the absence of endonuclease and exonuclease activity, with both double- and single-stranded DNA |
| Form | Supplied as a liquid in 20mM Tris-HCl, pH 7.5, 50mM KCl, 0.1mM EDTA, 5mM DTT, 50% glycerol. |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
