Thrombin, active, Human Plasma
559.21 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-T0203
Supplier
US Biologicals
Size
250U
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Thrombin enzyme (Activated Factor IIa) is an important clotting promoter that controls the transformation of soluble fibrinogen to insoluble active fibrin strands. Thrombin is a coagulation protein and a serine protease (EC 3.4.21.5) that catalyzes many coagulation-related reactions. Thrombin triggers factor-XI, factor-V, Factor-XIII and factor-VIII. Thrombin endorses platelet activation, using activation of protease-activated receptors on the platelet. As a result of its high proteolytic specificity, thrombin has become an important biochemical protein. The thrombin cleavage site (Leu-Val-Pro-Arg-Gly-Ser) is widely used in linker regions of recombinant fusion protein constructs. After the purification of the fusion protein, thrombin is used to cleave between the Arginine and Glycine residues of the cleavage site, efficiently removing the purification tag from the protein of interest with a high degree of specificity. Source: Human Plasma Molecular Weight: ~37kD Biological Activity: >3000units/mg protein Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Human Plasma |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 37 |
Product Properties
| Storage | -20°C |
| Grade | Highly Purified |
| Purity | ~95% (SDS-PAGE) |
| Form | Supplied as a lyophilized powder in 20mM Bis-Tris, 150mM NaCl, 0.1% PEG 8000, pH 6.5. Reconstitute with 1ml buffered saline, pH 7.4. |
| Reactivity Life | 6 months |
| Note | For reserch purpose only |
Target Gene
NA
