Transglutaminase, Tissue, Recombinant, Human (tTG)
419.70 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-T8259-98
Supplier
US Biologicals
Size
5 µg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Celiac disease is an enteropathy that is characterized by intestinal lesions of variable severity. Tissue-type transglutaminase (tTG) is believed to be the predominant autoantigen for celiac disease and the corresponding autoantibodies show higher sensitivity and specificity than anti-gliadin antibodies. Highly pure recombinant human tTG is now available to replace the traditionally used tTG fraction from guinea pig. Tissue-type transglutaminase antigens have been specifically modified for improved handling: exchange of an active site amino acid eliminates the protein cross-linking activity of the enzyme, while maintaining the native three-dimensional structure and the enzyme’s secondary GTPase activity. This engineering assures reproducible properties of the antigen preparations through the absence of variable and ill-defined covalent aggregates of tTG antigen and host cell proteins. Recombinant Human Tissue Transglutaminase produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 78.02kD. rHutTG is expressed with a -6xHis tag and purified by proprietary chromatographic techniques. By point mutation of the active center the catalytic transglutaminase activity has been eliminated, resulting in increased stability during storage and coating. Applications: Suitable for use in Western Blot with monoclonal anti-hexa-His-tag antibody and standard ELISA (checker-board analysis of positive/negative sera panels, immuno-dot test). Also suitable for biotinylation and iodination. Other applications not tested. Recommended Dilutions: ELISA: 0.6-1.4ug/ml (depending on the type of plate and coating buffer). Optimal dilutions to be determined by the researcher. Specificity: Binds IgG-type human auto-antibodies. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Recombinant, Sf9 insect cells |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 78.02 |
Product Properties
| Storage | -20°C |
| Grade | Highly Purified |
| Purity | ≥95% (SDS-PAGE) |
| Form | Supplied as a liquid in 16mM HEPES buffer, pH 7.6, 240mM sodium chloride and 20% glycerol. |
| Reactivity Life | 6 months |
| Note | For reserch purpose only |
Target Gene
NA
