Troponin-I, Skeletal Muscle, Monkey
568.05 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-209278
Supplier
US Biologicals
Size
25 µg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Troponins are protein components of striated muscle. There are three different troponins: troponin C, troponin T and troponin I. Troponin T (cTnT) and troponin I (cTnI) are released only following cardiac damage They are present for, and remain elevated, a long time Unlike CK and CK-MB, cTnT and cTnI are released for much longer with cTnI detectable in the blood for up to 5 days and cTnT for 7-10 days following MI. This allows an MI to be detected if the patient presents late. Troponin T and I are very sensitive. There is always a low level release of CK and CK-MB from skeletal muscle at a low level all the time so there is always a background value. This is not the case for the cardiac structural proteins such as cTnT and cTnI and therefore, they are very sensitive. Studies have revealed that about one third of patients admitted with unstable angina, in which MI was apparently excluded by CK and CK-MB measurement, have raised levels of cTnT and cTnI. Troponin C, skeletal muscle is a protein that in humans is encoded by the TNNC2 gene. Troponin (Tn), a key protein complex in the regulation of striated muscle contraction, is composed of 3 subunits. The Tn-I subunit inhibits actomyosin ATPase, the Tn-T subunit binds tropomyosin and Tn-C, while the Tn-C subunit binds calcium and overcomes the inhibitory action of the troponin complex on actin filaments. The protein encoded by this gene is the Tn-C subunit. cTnI is a part of the troponin complex. It binds to actin in thin myofilaments to hold the actin-tropomyosin complex in place. Because of it myosin cannot bind actin in relaxed muscle. When calcium binds to the Troponin C it causes conformational changes which lead to dislocation of troponin I and finally tropomyosin leaves the binding site for myosin on actin leading to contraction of muscle. The letter I is given due to its inhibitory character. Source: Troponin-I, from African green monkey skeletal muscle Molecular Weight: ~21kD Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Source Antigen | African green Monkey skeletal muscle |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 21 |
Product Properties
| Storage | -20°C |
| Grade | Highly Purified |
| Purity | Highly Purified (~95%) |
| Form | Supplied as a liquid in 50mM Tris, pH 8.0, 2mM EDTA, 8M urea, 5mM beta-mercaptoethanol. |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
