UbcH1, Recombinant, Human, His-Tag (Huntingtin Interacting Protein 2, HIP2, HIP-2)
530.03 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-209289
Supplier
US Biologicals
Size
10 µg
Product Overview
Product Properties
Target Gene
Product Overview
| Description | Among ubiquitin-conjugating enzymes, the mammalian ubiquitin conjugating enzyme UbcH1, also known as HIP2, is unique in its ability to catalyze the in vitro synthesis of unanchored Lys48-linked poly-ubiquitin chains from mono- or poly-ubiquitin, E1, and ATP. In addition, UbcH1 can catalyse the cyclization of longer poly-ubiquitin chains, including tetra- and penta-ubiquitin. Recombinant UbcH1 charges and supports ubiquitinylation in vitro. Typical enzyme concentration to support conjugation in vitro is 100nM-1uM. Recently, HIP2 (or UbcH1) has been shown to bind to the N-terminus of Huntington and may play a role in Huntington disease. Source: Recombinant protein corresponding to human UbcH1, a non-glycosylated, polypeptide chain containing 208aa, contains 6xHis-tag, expressed in E. coli. Molecular Weight: ~23.4kD Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
| Source Antigen | Recombinant, E. coli |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 23.4 |
Product Properties
| Storage | -20°C |
| Grade | Highly Purified |
| Purity | ~95% (RP-HPLC, SDS-PAGE) |
| Form | Supplied as a lyophilized powder in PBS, 1mM DTT, pH 7.5. Reconstitute with sterile ddH2O >100ug/ml. |
| Reactivity Life | 12 months |
| Note | For reserch purpose only |
Target Gene
NA
