Urease, Recombinant discontinued
607.94 $
For reserch purpose only
Shipping cost plus VAT not included , delivery in 7-14 business days
Catalog No
USB-U2015-01A
Supplier
US Biologicals
Size
1000U
Product Overview
Product Properties
Target Gene
Product Overview
| Description | The active mutant urease is expressed in E. coli by recombinant DNA procedures and purified by conventional chromatographies. Mutant urease from Klebsiella aerogenes has a vast Km value (200mM). The substitution of His219 at the active center of a subunit to other amino acid residues by protein engineering is known to modify the affinity against urea and to shift Km. Among those mutants, aH219Q has almost similar enzymatic properities except for a vast Km against urea. The Km value is 200mM, being 1000 fold larger than that of wild type (0.2mM). Reaction Equation: Urea + H2O + 2H+ = 2NH4+ +CO2 Biological Activity: As reported Specific Activity: ~150u/mg protein (25°C, pH 7.0). Standard: Recombinant Urease-03 Contaminant: (as Recombinant Urease activity = 100%) NADPHox ≤ 0.001% One mg of powder contains the following: Protein 500 ± 300ug K-PO4 470 ± 300ug EDTA•Na2 30 ± 10ug pH Stability: pH 8.0-9.5 (37°C, 1 week) Thermal Stability: ≥65°C (pH 8.0, 10 min) Optimum pH: 6.0 Optimum Temperature: ≥37°C MW: 60.3kD alpja subunit, 11.7kD beta subunit, 11.1kD gamma subunit (SDS-PAGE) Application: Suitable for use as a calibrator or control serum. Other applications not tested. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
| Source Antigen | Recombinant, E. coli |
| Reactivity | |
| Cross Reactivity | |
| Applications | |
| Molecular Weight | 224 |
Product Properties
| Storage | -20°C |
| Grade | Purified |
| Purity | |
| Form | Supplied as lyophilized powder. |
| Reactivity Life | 6 months |
| Note | For reserch purpose only |
Target Gene
NA
